Lsm12 Mediates Deubiquitination of DNA Polymerase ? To Help Saccharomyces cerevisiae Resist Oxidative Stress.

ABSTRACT: In Saccharomyces cerevisiae, the Y family DNA polymerase ? (Pol?) regulates genome stability in response to different forms of environmental stress by translesion DNA synthesis. To elucidate the role of Pol? in oxidative stress-induced DNA damage, we deleted or overexpressed the corresponding gene RAD30 and used transcriptome analysis to screen the potential genes associated with RAD30 to respond to DNA damage. Under 2 mM H2O2 treatment, the deletion of RAD30 resulted in a 2.2-fold decrease in survival and a 2.8-fold increase in DNA damage, whereas overexpression of RAD30 increased survival and decreased DNA damage by 1.2- and 1.4-fold, respectively, compared with the wild-type strain. Transcriptome and phenotypic analyses identified Lsm12 as a main factor involved in oxidative stress-induced DNA damage. Deleting LSM12 caused growth defects, while its overexpression enhanced cell growth under 2?mM H2O2 treatment. This effect was due to the physical interaction of Lsm12 with the UBZ domain of Pol? to enhance Pol? deubiquitination through Ubp3 and consequently promote Pol? recruitment. Overall, these findings demonstrate that Lsm12 is a novel regulator mediating Pol? deubiquitination to promote its recruitment under oxidative stress. Furthermore, this study provides a potential strategy to maintain the genome stability of industrial strains during fermentation.IMPORTANCE Pol? was shown to be critical for cell growth in the yeast Saccharomyces cerevisiae, and deletion of its corresponding gene RAD30 caused a severe growth defect under exposure to oxidative stress with 2 mM H2O2 Furthermore, we found that Lsm12 physically interacts with Pol? and promotes Pol? deubiquitination and recruitment. Overall, these findings indicate Lsm12 is a novel regulator mediating Pol? deubiquitination that regulates its recruitment in response to DNA damage induced by oxidative stress.

SUBMITTER: Yao R 

PROVIDER: S-EPMC6293111 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature