Dataset Information

Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium.

ABSTRACT: Cardiomyocytes (CMs) generated from human induced pluripotent stem cells (hiPSCs) are under investigation for their suitability as human models in preclinical drug development. Antiarrhythmic drug development focuses on atrial biology for the treatment of atrial fibrillation. Here we used recent retinoic acid-based protocols to generate atrial CMs from hiPSCs and establish right atrial engineered heart tissue (RA-EHT) as a 3D model of human atrium. EHT from standard protocol-derived hiPSC-CMs (Ctrl-EHT) and intact human muscle strips served as comparators. RA-EHT exhibited higher mRNA and protein concentrations of atrial-selective markers, faster contraction kinetics, lower force generation, shorter action potential duration, and higher repolarization fraction than Ctrl-EHTs. In addition, RA-EHTs but not Ctrl-EHTs responded to pharmacological manipulation of atrial-selective potassium currents. RA- and Ctrl-EHTs' behavior reflected differences between human atrial and ventricular muscle preparations. Taken together, RA-EHT is a model of human atrium that may be useful in preclinical drug screening.

SUBMITTER: Lemme M

PROVIDER: S-EPMC6294072 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium.

Lemme Marta M Ulmer Bärbel M BM Lemoine Marc D MD Zech Antonia T L ATL Flenner Frederik F Ravens Ursula U Reichenspurner Hermann H Rol-Garcia Miriam M Smith Godfrey G Hansen Arne A Christ Torsten T Eschenhagen Thomas T

Stem cell reports 20181108 6

Cardiomyocytes (CMs) generated from human induced pluripotent stem cells (hiPSCs) are under investigation for their suitability as human models in preclinical drug development. Antiarrhythmic drug development focuses on atrial biology for the treatment of atrial fibrillation. Here we used recent retinoic acid-based protocols to generate atrial CMs from hiPSCs and establish right atrial engineered heart tissue (RA-EHT) as a 3D model of human atrium. EHT from standard protocol-derived hiPSC-CMs (C ...[more]

PMID: 30416051

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Dataset Information

Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium.

Publications

Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium.

Similar Datasets

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