Dataset Information

Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action.

ABSTRACT:

Background

Epigenetic modifications including DNA methylation and post-translational modifications of histones are known to regulate gene expression. Antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) mediate transcriptional reprogramming during insect development as shown in Drosophila melanogaster and other insects. Juvenile hormones (JH) play vital roles in the regulation of growth, development, metamorphosis, reproduction and other physiological processes. However, our current understanding of epigenetic regulation of JH action is still limited. Hence, we studied the role of CREB binding protein (CBP, contains HAT domain) and Trichostatin A (TSA, HDAC inhibitor) on JH action.

Results

Exposure of Tribolium castaneum cells (TcA cells) to JH or TSA caused an increase in expression of Kr-h1 (a known JH-response gene) and 31 or 698 other genes respectively. Knockdown of the gene coding for CBP caused a decrease in the expression of 456 genes including Kr-h1. Interestingly, the expression of several genes coding for transcription factors, nuclear receptors, P450 and fatty acid synthase family members that are known to mediate JH action were affected by CBP knockdown or TSA treatment.

Conclusions

These data suggest that acetylation and deacetylation mediated by HATs and HDACs play an important role in JH action.

SUBMITTER: Roy A

PROVIDER: S-EPMC6295036 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Publications

Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action.

Roy Amit A Palli Subba Reddy SR

BMC genomics 20181214 1

<h4>Background</h4>Epigenetic modifications including DNA methylation and post-translational modifications of histones are known to regulate gene expression. Antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) mediate transcriptional reprogramming during insect development as shown in Drosophila melanogaster and other insects. Juvenile hormones (JH) play vital roles in the regulation of growth, development, metamorphosis, reproduction and other physiolog ...[more]

PMID: 30547764

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Dataset Information

Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action.

Background

Results

Conclusions

Publications

Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action.

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