Dataset Information

The urinary microbiome in women with mixed urinary incontinence compared to similarly aged controls.

ABSTRACT:

Introduction & hypothesis

Previous studies have suggested that women with urinary incontinence have an altered urinary microbiome. We hypothesized that the microbiome in women with mixed urinary incontinence (MUI) differed from controls and tested this hypothesis using bacterial gene sequencing techniques.

Methods

This multicenter study compared the urinary microbiome in women with MUI and similarly aged controls. Catheterized urine samples were obtained; v4-6 regions of the 16S rRNA gene were sequenced to identify bacteria. Bacterial predominance (> 50% of an individual's genera) was compared between MUI and controls. Bacterial sequences were categorized into "community types" using Dirichlet multinomial mixture (DMM) methods. Generalized linear mixed models predicted MUI/control status based on clinical characteristics and community type. Post-hoc analyses were performed in women < 51 and ≥ 51 years. Sample size estimates required 200 samples to detect a 20% difference in Lactobacillus predominance with P < 0.05.

Results

Of 212 samples, 97.6% were analyzed (123 MUI/84 controls, mean age 53 ± 11 years). Overall Lactobacillus predominance did not differ between MUI and controls (45/123 = 36.6% vs. 36/84 = 42.9%, P = 0.36). DMM analyses revealed six community types; communities differed by age (P = 0.001). A High-Lactobacillus (89.2% Lactobacillus) community had a greater proportion of controls (19/84 = 22.6%, MUI 11/123 = 8.9%). Overall, bacterial community types did not differ in MUI and controls. However, post-hoc analysis of women < 51 years found that bacterial community types distinguished MUI from controls (P = 0.041); Moderate-Lactobacillus (aOR 7.78, CI 1.85-32.62) and Mixed (aOR 7.10, CI 1.32-38.10) community types were associated with MUI. Community types did not differentiate MUI and controls in women ≥ 51 years (P = 0.94).

Conclusions

Women with MUI and controls did not differ in overall Lactobacillus predominance. In younger women, urinary bacterial community types differentiated MUI from controls.

SUBMITTER: Komesu YM

PROVIDER: S-EPMC6295358 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Publications

The urinary microbiome in women with mixed urinary incontinence compared to similarly aged controls.

Komesu Yuko M YM Richter Holly E HE Carper Benjamin B Dinwiddie Darrell L DL Lukacz Emily S ES Siddiqui Nazema Y NY Sung Vivian W VW Zyczynski Halina M HM Ridgeway Beri B Rogers Rebecca G RG Arya Lily A LA Mazloomdoost Donna D Gantz Marie G MG

International urogynecology journal 20180616 12

<h4>Introduction & hypothesis</h4>Previous studies have suggested that women with urinary incontinence have an altered urinary microbiome. We hypothesized that the microbiome in women with mixed urinary incontinence (MUI) differed from controls and tested this hypothesis using bacterial gene sequencing techniques.<h4>Methods</h4>This multicenter study compared the urinary microbiome in women with MUI and similarly aged controls. Catheterized urine samples were obtained; v4-6 regions of the 16S r ...[more]

PMID: 29909556

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Dataset Information

The urinary microbiome in women with mixed urinary incontinence compared to similarly aged controls.

Introduction & hypothesis

Methods

Results

Conclusions

Publications

The urinary microbiome in women with mixed urinary incontinence compared to similarly aged controls.

Similar Datasets

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