The urinary microbiome in women with mixed urinary incontinence compared to similarly aged controls.

ABSTRACT: INTRODUCTION & HYPOTHESIS:Previous studies have suggested that women with urinary incontinence have an altered urinary microbiome. We hypothesized that the microbiome in women with mixed urinary incontinence (MUI) differed from controls and tested this hypothesis using bacterial gene sequencing techniques. METHODS:This multicenter study compared the urinary microbiome in women with MUI and similarly aged controls. Catheterized urine samples were obtained; v4-6 regions of the 16S rRNA gene were sequenced to identify bacteria. Bacterial predominance (> 50% of an individual's genera) was compared between MUI and controls. Bacterial sequences were categorized into "community types" using Dirichlet multinomial mixture (DMM) methods. Generalized linear mixed models predicted MUI/control status based on clinical characteristics and community type. Post-hoc analyses were performed in women < 51 and???51 years. Sample size estimates required 200 samples to detect a 20% difference in Lactobacillus predominance with P?

SUBMITTER: Komesu YM 

PROVIDER: S-EPMC6295358 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature