Dataset Information

BrainEXP: a database featuring with spatiotemporal expression variations and co-expression organizations in human brains.

ABSTRACT:

Summary

Gene expression changes over the lifespan and varies among different tissues or cell types. Gene co-expression also changes by sex, age, different tissues or cell types. However, gene expression under the normal state and gene co-expression in the human brain has not been fully defined and quantified. Here we present a database named Brain EXPression Database (BrainEXP) which provides spatiotemporal expression of individual genes and co-expression in normal human brains. BrainEXP consists of 4567 samples from 2863 healthy individuals gathered from existing public databases and our own data, in either microarray or RNA-Seq library types. We mainly provide two analysis results based on the large dataset: (i) basic gene expression across specific brain regions, age ranges and sexes; (ii) co-expression analysis from different platforms.

Availability and implementation

http://www.brainexp.org/.

Supplementary information

Supplementary data are available at Bioinformatics online.

SUBMITTER: Jiao C

PROVIDER: S-EPMC6298050 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Publications

BrainEXP: a database featuring with spatiotemporal expression variations and co-expression organizations in human brains.

Jiao Chuan C Yan Pengpeng P Xia Cuihua C Shen Zhaoming Z Tan Zexi Z Tan Yanyan Y Wang Kangli K Jiang Yi Y Huang Lingling L Dai Rujia R Wei Yu Y Xia Yan Y Meng Qingtuan Q Ouyang Yanmei Y Yi Liu L Duan Fangyuan F Dai Jiacheng J Zhao Shunan S Liu Chunyu C Chen Chao C

Bioinformatics (Oxford, England) 20190101 1

<h4>Summary</h4>Gene expression changes over the lifespan and varies among different tissues or cell types. Gene co-expression also changes by sex, age, different tissues or cell types. However, gene expression under the normal state and gene co-expression in the human brain has not been fully defined and quantified. Here we present a database named Brain EXPression Database (BrainEXP) which provides spatiotemporal expression of individual genes and co-expression in normal human brains. BrainEXP ...[more]

PMID: 29985970

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Project description:Flexor tendinopathy is a common problem affecting humans and animals. Tendon healing is poorly understood and the outcomes of conservative and surgical management are often suboptimal. While often considered a localized injury, recent evidence indicates that in the short term, tendinopathic changes are distributed widely throughout the tendon, remote from the lesion itself. Whether these changes persist throughout healing is unknown. The aim of this study was to document gene expression, histopathological and biomechanical changes that occur throughout the superficial digital flexor tendon (SDFT) up to 16 weeks post-injury, using an ovine surgical model of tendinopathy. Partial tendon transection was associated with decreased gene expression for aggrecan, decorin, fibromodulin, tissue inhibitors of metalloproteinases (TIMPS 1, 2 and 3), collagen I and collagen II. Gene expression for collagen III, lumican and matrix metalloproteinase 13 (MMP13) increased locally around the lesion site. Expression of collagen III and MMP13 decreased with time, but compared to controls, collagen III, MMP13 and lumican expression remained regionally high throughout the study. An increase in TIMP3 was observed over time. Histologically, operated tendons had higher pathology scores than controls, especially around the injured region. A chondroid phenotype was observed with increased cellular rounding and marked proteoglycan accumulation which only partially improved with time. Biomechanically, partial tendon transection resulted in a localized decrease in elastic modulus (in compression) but only at 8 weeks postoperatively. This study improves our understanding of tendon healing, demonstrating an early 'peak' in pathology characterized by altered gene expression and notable histopathological changes. Many of these pathological changes become more localized to the region of injury during healing. Collagen III and MMP13 expression levels remained high close to the lesion throughout the study and may reflect the production of tendon tissue with suboptimal biomechanical properties. Further studies evaluating the long-term response of tendon to injury (6-12 months) are warranted to provide additional information on tendon healing and provide further understanding of the mechanisms underlying the pathology observed in this study.

Dataset Information

BrainEXP: a database featuring with spatiotemporal expression variations and co-expression organizations in human brains.

Summary

Availability and implementation

Supplementary information

Publications

BrainEXP: a database featuring with spatiotemporal expression variations and co-expression organizations in human brains.

Similar Datasets

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