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Sensory and optogenetically driven single-vessel fMRI.

ABSTRACT: Magnetic resonance imaging (MRI) sensitivity approaches vessel specificity. We developed a single-vessel functional MRI (fMRI) method to image the contribution of vascular components to blood oxygenation level-dependent (BOLD) and cerebral blood volume (CBV) fMRI signal. We mapped individual vessels penetrating the rat somatosensory cortex with 100-ms temporal resolution by MRI with sensory or optogenetic stimulation. The BOLD signal originated primarily from venules, and the CBV signal from arterioles. The single-vessel fMRI method and its combination with optogenetics provide a platform for mapping the hemodynamic signal through the neurovascular network with specificity at the level of individual arterioles and venules.

SUBMITTER: Yu X

PROVIDER: S-EPMC6298439 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Sensory and optogenetically driven single-vessel fMRI.

Yu Xin X He Yi Y Wang Maosen M Merkle Hellmut H Dodd Stephen J SJ Silva Afonso C AC Koretsky Alan P AP

Nature methods 20160208 4

Magnetic resonance imaging (MRI) sensitivity approaches vessel specificity. We developed a single-vessel functional MRI (fMRI) method to image the contribution of vascular components to blood oxygenation level-dependent (BOLD) and cerebral blood volume (CBV) fMRI signal. We mapped individual vessels penetrating the rat somatosensory cortex with 100-ms temporal resolution by MRI with sensory or optogenetic stimulation. The BOLD signal originated primarily from venules, and the CBV signal from art ...[more]

PMID: 26855362

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