Unknown

Dataset Information

0

Superior effect of MP-AzeFlu than azelastine or fluticasone propionate alone on reducing inflammatory markers.


ABSTRACT: Background:MP-AzeFlu, intranasal formulation of azelastine hydrochloride (AZE) and fluticasone propionate (FP), is superior to AZE or FP alone for treatment of allergic rhinitis (AR). However, the precise anti-inflammatory mechanism of action of MP-AzeFlu has not been characterized. Objective:To investigate the anti-inflammatory effects of MP-AzeFlu compared with AZE or FP alone in an established in vitro model of eosinophilic inflammation. Methods:Nasal mucosal epithelial cells and peripheral blood eosinophils were obtained from human volunteers. Epithelial cells were stimulated with 10% fetal bovine serum (FBS) in the presence of MP-AzeFlu, AZE, or FP (1:102 to 1:105 dilution). Concentrations of interleukin (IL)-6, IL-8, and granulocyte-macrophage colony-stimulating factor (GM-CSF) were measured by ELISA. Eosinophils were incubated in 10% human epithelial cell-conditioned medium (HECM) and survival assessed by trypan blue dye exclusion. Results are expressed as mean?±?SEM percentage secretion/survival compared with FBS/HECM (respectively). Results:FP and MP-AzeFlu (all dilutions) and AZE (1:102) significantly reduced IL-6 secretion and eosinophil survival compared with positive controls. At 1:102 dilution, IL-6 secretion was significantly lower with MP-AzeFlu (38.3?±?4.2%, compared with FBS?=?100%) than with AZE (76.1?±?4.9%) or FP (53.0?±?4.9%). At 1:102 dilution, eosinophil survival was significantly lower with MP-AzeFlu at day 3 (17.5?±?3.0%) and day 4 (2.4?±?1.4%, compared with HECM?=?100%) than with AZE (day 3: 75.2?±?7.2%; day 4: 44.0?±?9.7%) or FP (day 3: 38.5?±?3.5%; day 4: 14.6?±?4.0%). Conclusion:Greater reductions in cytokine secretion and eosinophil survival observed with MP-AzeFlu in vitro may underlie MP-AzeFlu's superior clinical efficacy vs. AZE or FP alone observed in AR patients.

SUBMITTER: Roca-Ferrer J 

PROVIDER: S-EPMC6299636 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

altmetric image

Publications

Superior effect of MP-AzeFlu than azelastine or fluticasone propionate alone on reducing inflammatory markers.

Roca-Ferrer Jordi J   Pujols Laura L   Pérez-González Maria M   Alobid Isam I   Callejas Borja B   Vicens-Artés Sònia S   Fuentes Mireya M   Valero Antonio A   Picado César C   Castor Dennis D   Nguyen DucTung D   Mullol Joaquim J  

Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 20181218


<h4>Background</h4>MP-AzeFlu, intranasal formulation of azelastine hydrochloride (AZE) and fluticasone propionate (FP), is superior to AZE or FP alone for treatment of allergic rhinitis (AR). However, the precise anti-inflammatory mechanism of action of MP-AzeFlu has not been characterized.<h4>Objective</h4>To investigate the anti-inflammatory effects of MP-AzeFlu compared with AZE or FP alone in an established in vitro model of eosinophilic inflammation.<h4>Methods</h4>Nasal mucosal epithelial  ...[more]

Similar Datasets

| S-EPMC8926499 | biostudies-literature
| S-EPMC5827154 | biostudies-literature
| S-EPMC3394136 | biostudies-literature
| S-EPMC8278066 | biostudies-literature
| S-EPMC5121625 | biostudies-literature
| S-EPMC3620714 | biostudies-literature
| S-EPMC3426464 | biostudies-literature
| S-EPMC4545560 | biostudies-literature
| S-EPMC4407765 | biostudies-literature