Dataset Information

In vitro study of probiotic properties of Lactobacillus plantarum F22 isolated from chhang - A traditional fermented beverage of Himachal Pradesh, India.

ABSTRACT: Present study was carried out to evaluate a new bacterial strain, Lactobacillus plantarum F22 as probiotic strain. L. plantarum F22 was isolated from a traditional inoculum called 'Phab' which is used for the preparation of a traditional beverage chhang of Lahaul and Spiti of Himachal Pradesh. The isolate was identified by conventional and molecular techniques and tested for different probiotic properties. The 16S rRNA sequence of the isolate was registered in National Centre for Biotechnology Information (NCBI) under accession number KT865223. Further, L. plantarum F22 was evaluated for its probiotic potential viz., autoaggregation capacity, hydrophobicity, acidity tolerance, antibiotic susceptibility and cumulative probiotic potential and was found to possess good probiotic potential with a cumulative probiotic score of 91.7%. L. plantarum F22 has been proved to be highly effective, therefore can be recommended for the development of new pharmaceuticals and functional food preparations.

SUBMITTER: Handa S

PROVIDER: S-EPMC6300045 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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In vitro study of probiotic properties of Lactobacillus plantarum F22 isolated from chhang - A traditional fermented beverage of Himachal Pradesh, India.

Handa Shweta S Sharma Nivedita N

Journal, genetic engineering & biotechnology 20160601 1

Present study was carried out to evaluate a new bacterial strain, Lactobacillus plantarum F22 as probiotic strain. L. plantarum F22 was isolated from a traditional inoculum called 'Phab' which is used for the preparation of a traditional beverage chhang of Lahaul and Spiti of Himachal Pradesh. The isolate was identified by conventional and molecular techniques and tested for different probiotic properties. The 16S rRNA sequence of the isolate was registered in National Centre for B ...[more]

PMID: 30647602

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Project description:Lactic acid bacteria (LAB) are indigenous microorganisms that have been involved in food fermentations throughout history to preserve food and also to give special characteristics to them. The traditional fermented foods that are still being elaborated in indigenous populations around the world are a potential source of LAB with important biotechnological properties and/or beneficial to health. In a previous work, LAB biodiversity associated with chicha, a traditional maize-based fermented beverage from Northwestern Argentina, was studied, both by culture dependent and independent methods. From that study, 392 isolates were recovered, mostly members of Lactobacillus and Leuconostoc. Biotechnological characterization of representative isolates led to the selection of five strains belonging to the species Lactobacillus plantarum for their ability to produce vitamin B2 (riboflavin) and vitamin B9 (folates), their antimicrobial properties and antibiotics susceptibility. In this work, we present the Whole Genome Sequences (WGS) of these five strains that have been deposited in the Spanish Type Culture Collection: M5MA1 (= CECT 8962), M9MM1 (= CECT 8963), M9MM4 (= CECT 8964), M9MG6 (= CECT 8965), and M9Y2 (= CECT 8966), and a detailed description of their characterization, through a genomic approach, analyzing the genes responsible for these biotechnological properties, making a comparative study of the five genomes and reporting the aspects related to food safety, in accordance with the recommendations of the European Food Safety Authority (EFSA FEEDAP Panel, 2018) aiming at their use in the design of functional foods. The analysis unveiled, for the five strains, the complete set of genes for folate and riboflavin biosynthesis, the absence of pathogenic factors, the presence of CRISPR and cas genes, phage sequences, insertion elements and an aminoglycosides resistance gene, aadA, whose resistance could not be proved phenotypically in any strain. Genomic comparisons showed that strain CECT 8962 was significantly different in terms of genetic content and allowed the identification of carbohydrates metabolism and membrane transport related genes as the main components of the unique and accessory genome.

Dataset Information

In vitro study of probiotic properties of Lactobacillus plantarum F22 isolated from chhang - A traditional fermented beverage of Himachal Pradesh, India.

Publications

<i>In vitro</i> study of probiotic properties of <i>Lactobacillus plantarum</i> F22 isolated from chhang - A traditional fermented beverage of Himachal Pradesh, India.

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