Relative Quantitation of Polymerized Actin in Suspension Cells by Flow Cytometry.
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ABSTRACT:
SUBMITTER: Kakley MR
PROVIDER: S-EPMC6301187 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Publications
Relative Quantitation of Polymerized Actin in Suspension Cells by Flow Cytometry.
Kakley Mallory R MR Velle Katrina B KB Fritz-Laylin Lillian K LK
Bio-protocol 20181101 22
The amount of polymerized actin within a cell can vary widely due to natural processes and/or experimentally induced perturbations. We routinely use this protocol to measure relative polymerized actin content between cell populations by staining cells in suspension with fluorescent phalloidin, then measuring total cell fluorescence using flow cytometry. Although developed for human cells, we have easily adapted this method for use with diverse eukaryotic cell types. ...[more]
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