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Cherenkov luminescence imaging is a fast and relevant preclinical tool to assess tumour hypoxia in vivo.

ABSTRACT: PURPOSE:Molecular imaging techniques visualise biomarkers for both drug development and personalised medicine. In this field, Cherenkov luminescence imaging (CLI) seems to be very attractive by allowing imaging with clinical PET radiotracers with high-throughput capabilities. In this context, we developed a fast CLI method to detect tumour hypoxia with 18F-fluoromisonidazole (FMISO) for drug development purposes. METHODS:Colon cancer model was induced in mice by subcutaneous injection of 1?×?106 CT-26 cells. FMISO was injected, and simultaneous PET-blood oxygen level dependent (BOLD)-MRI followed by CLI were performed along with immunohistochemistry staining with pimonidazole. RESULTS:There was a significant correlation between FMISO PET and CLI tumour uptakes, consistent with the BOLD-MRI mapping. Tumour-to-background ratio was significantly higher for CLI compared with PET and MRI. Immunohistochemistry confirmed tumour hypoxia. The imaging workflow with CLI was about eight times faster than the PET-MRI procedure. CONCLUSION:CLI is a fast and relevant tool to assess tumour hypoxia. This approach could be particularly interesting for hypoxia-targeting drug development.

SUBMITTER: Desvaux E

PROVIDER: S-EPMC6301908 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Cherenkov luminescence imaging is a fast and relevant preclinical tool to assess tumour hypoxia in vivo.

Desvaux Emiko E Courteau Alan A Bellaye Pierre-Simon PS Guillemin Mélanie M Drouet Camille C Walker Paul P Collin Bertrand B Decréau Richard A RA

EJNMMI research 20181220 1

<h4>Purpose</h4>Molecular imaging techniques visualise biomarkers for both drug development and personalised medicine. In this field, Cherenkov luminescence imaging (CLI) seems to be very attractive by allowing imaging with clinical PET radiotracers with high-throughput capabilities. In this context, we developed a fast CLI method to detect tumour hypoxia with <sup>18</sup>F-fluoromisonidazole (FMISO) for drug development purposes.<h4>Methods</h4>Colon cancer model was induced in mice by subcuta ...[more]

PMID: 30574662

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Project description:BackgroundPhysical inactivity and unhealthy diet are key behavioral determinants underlying obesity. The neighborhood environment represents an important arena for modifying these behaviors, and hence reliable and valid tools to measure it are needed. Most existing virtual audit tools have been designed to assess either food or activity environments deemed relevant for adults. Thus, there is a need for a tool that combines the assessment of food and activity environments, and which focuses on aspects of the environment relevant for youth.ObjectiveThe aims of the present study were: (a) to adapt the SPOTLIGHT Virtual Audit Tool (S-VAT) developed to assess characteristics of the built environment deemed relevant for adults for use in an adolescent population, (b) to assess the tool's inter- and intra-rater reliability, and (c) to assess its criterion validity by comparing the virtual audit to a field audit.MethodsThe tool adaptation was based on literature review and on results of a qualitative survey investigating how adolescents perceived the influence of the environment on dietary and physical activity behaviors. Sixty streets (148 street segments) in six neighborhoods were randomly selected as the study sample. Two raters assessed the inter- and intra-rater reliability and criterion validity, comparing the virtual audit tool to a field audit. The results were presented as percentage agreement and Cohen's kappa (κ).ResultsIntra-rater agreement was found to be moderate to almost perfect (κ = 0.44-0.96) in all categories, except in the category aesthetics (κ = 0.40). Inter-rater agreement between auditors ranged from fair to substantial for all categories (κ = 0.24-0.80). Criterion validity was found to be moderate to almost perfect (κ = 0.56-0.82) for most categories, except aesthetics and grocery stores (κ = 0.26-0.35).ConclusionThe adapted version of the S-VAT can be used to provide reliable and valid data on built environment characteristics deemed relevant for physical activity and dietary behavior among adolescents.

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Cherenkov luminescence imaging is a fast and relevant preclinical tool to assess tumour hypoxia in vivo.

Publications

Cherenkov luminescence imaging is a fast and relevant preclinical tool to assess tumour hypoxia in vivo.

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