Dataset Information

Spleen-Kidney Supplementing Formula Alleviates Renal Fibrosis in Diabetic Rats via TGF-?1-miR-21-PTEN Signaling Pathway.

ABSTRACT:

Background

Spleen-Kidney Supplementing Formula (SKSF), composed of 6 raw Chinese herbs and proposed based on the therapeutic principle of supplementing spleen-kidney and clearing the extra heat of stomach-lung, is effective in the treatment of type 2 diabetes mellitus (T2DM).

Aim

This study aims to investigate the mechanism of SKSF to alleviate renal fibrosis in diabetic rats.

Methods

T2DM model was induced by high-fat diet and multiple injections of low-dose streptozotocin. After 8-week intervention, samples were collected for detection.

Results

SKSF decreased fasting blood glucose, glycosylated hemoglobin A1c, blood urea nitrogen, uric acid, urea, 24-hour urine protein, and KW/BW ratio, while it increased creatinine clearance rate of T2DM rats. Meanwhile, SKSF attenuated the renal fibrosis and improved the morphology and structure of renal tissue. Furthermore, SKSF significantly reduced the expression level of plasma miR-21 and TGF-?1 protein level and increased PTEN protein level.

Conclusion

SKSF could attenuate the renal damage and renal fibrosis induced in T2DM, which may be related to its regulation on the expressions of TGF-?1, PTEN, and miR-21.

SUBMITTER: Tian C

PROVIDER: S-EPMC6304600 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Spleen-Kidney Supplementing Formula Alleviates Renal Fibrosis in Diabetic Rats via TGF-<i>β</i>1-miR-21-PTEN Signaling Pathway.

Tian Chunyu C Wang Ya Y Chang Hong H Li Ji'an J La Xiaojin X

Evidence-based complementary and alternative medicine : eCAM 20181206

<h4>Background</h4>Spleen-Kidney Supplementing Formula (SKSF), composed of 6 raw Chinese herbs and proposed based on the therapeutic principle of supplementing spleen-kidney and clearing the extra heat of stomach-lung, is effective in the treatment of type 2 diabetes mellitus (T2DM).<h4>Aim</h4>This study aims to investigate the mechanism of SKSF to alleviate renal fibrosis in diabetic rats.<h4>Methods</h4>T2DM model was induced by high-fat diet and multiple injections of low-dose streptozotocin ...[more]

PMID: 30622599

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