Ontology highlight
ABSTRACT:
SUBMITTER: Park JS
PROVIDER: S-EPMC6308251 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Park Joseph S JS Helble Jennifer D JD Lazarus Jacob E JE Yang Guanhua G Blondel Carlos J CJ Doench John G JG Starnbach Michael N MN Waldor Matthew K MK
iScience 20181214
The invasion of Chlamydia trachomatis, an obligate intracellular bacterium, into epithelial cells is driven by a complex interplay of host and bacterial factors. To comprehensively define the host genes required for pathogen invasion, we undertook a fluorescence-activated cell sorting (FACS)-based CRISPR screen in human cells. A genome-wide loss-of-function library was infected with fluorescent C. trachomatis and then sorted to enrich for invasion-deficient mutants. The screen identified heparan ...[more]