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Real-time crystallographic studies of the adenine riboswitch using an X-ray free-electron laser.

ABSTRACT: Structures of the four reaction states of the adenine riboswitch aptamer domain, including a transient intermediate state were solved by serial femtosecond crystallography. The structures not only demonstrate the use of X-ray free-electron lasers for RNA crystallography but have also proven that transient states can be determined in real time by mix-and-inject crystallography. These results illustrate the structural basis for the ligand-induced conformational changes associated with the molecular 'switch'.

SUBMITTER: Stagno JR

PROVIDER: S-EPMC6309305 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Real-time crystallographic studies of the adenine riboswitch using an X-ray free-electron laser.

Stagno Jason R JR Bhandari Yuba R YR Conrad Chelsie E CE Liu Yu Y Wang Yun-Xing YX

The FEBS journal 20170801 20

Structures of the four reaction states of the adenine riboswitch aptamer domain, including a transient intermediate state were solved by serial femtosecond crystallography. The structures not only demonstrate the use of X-ray free-electron lasers for RNA crystallography but have also proven that transient states can be determined in real time by mix-and-inject crystallography. These results illustrate the structural basis for the ligand-induced conformational changes associated with the molecula ...[more]

PMID: 28504865

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