Ontology highlight
ABSTRACT:
SUBMITTER: Li J
PROVIDER: S-EPMC6309308 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Li Jing J Zhang Yaru Y Da Silva Sil Dos Santos Bruno B Wang Feng F Ma Yuyong Y Perez Christian C Yang Yanling Y Peng Junmin J Cohen Seth M SM Chou Tsui-Fen TF Hilton Stephen T ST Deshaies Raymond J RJ
Cell chemical biology 20180823 11
The 26S proteasome is the major proteolytic machine for breaking down cytosolic and nuclear proteins in eukaryotes. Due to the lack of a suitable assay, it is difficult to measure routinely and quantitatively the breakdown of proteins by the 26S proteasome in vitro. In the present study, we developed an assay to monitor proteasome-mediated protein degradation. Using this assay, we discovered that epidithiodiketopiperazine (ETPs) blocked the degradation of our model substrate in vitro. Further ch ...[more]