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Quantifying single-cell secretion in real time using resonant hyperspectral imaging.

ABSTRACT: Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

SUBMITTER: Juan-Colas J

PROVIDER: S-EPMC6310807 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Quantifying single-cell secretion in real time using resonant hyperspectral imaging.

Juan-Colás José J Hitchcock Ian S IS Coles Mark M Johnson Steven S Krauss Thomas F TF

Proceedings of the National Academy of Sciences of the United States of America 20181210 52

Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin ...[more]

PMID: 30530663

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