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Biochemical and Biophysical Characterization of the Enolase from Helicobacter pylori.


ABSTRACT: Enolase, which catalyses the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate, is an important enzyme in the classic glycolysis pathway in cells. Enolase is highly conserved in organisms from bacteria to humans, indicating its importance in cells. Thus, enolase is a good target for developing new drugs. In the last decade, new functions of this enzyme have been found. Helicobacter pylori is a common human pathogen that causes gastric diseases and even gastric cancer. In this study, the sequence of H. pylori enolase (HpEno) was analysed; the conservation (at least partial) of binding sites for cofactor, plasminogen, and host extracellular RNA, as well as catalytic site, indicates that HpEno should be capable of performing the functions. Recombinant HpEno was overexpressed and purified from E. coli. Compared to the enolases from other species, HpEno had similar characteristics for its secondary structure. The temperature-induced profiles indicate that HpEno is quite stable to temperature, compared to other homologs. Regarding the kinetics of the unfolding reaction, we found that the activation enthalpy associated with the thermal unfolding reaction is equivalent to the reported activation enthalpy for yeast enolase, indicating a similar scaffold and kinetic stability. Although a wide range of experimental conditions were assayed, it was not possible to detect any enzymatic activity of HpEno. To prove the lack of activity, still a much wider range of experiments should be carried out.

SUBMITTER: Lopez-Lopez MJ 

PROVIDER: S-EPMC6311853 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Biochemical and Biophysical Characterization of the Enolase from <i>Helicobacter pylori</i>.

López-López María de J MJ   Rodríguez-Luna Isabel C IC   Lara-Ramírez Edgar E EE   López-Hidalgo Marisol M   Benítez-Cardoza Claudia G CG   Guo Xianwu X  

BioMed research international 20181217


Enolase, which catalyses the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate, is an important enzyme in the classic glycolysis pathway in cells. Enolase is highly conserved in organisms from bacteria to humans, indicating its importance in cells. Thus, enolase is a good target for developing new drugs. In the last decade, new functions of this enzyme have been found. <i>Helicobacter pylori</i> is a common human pathogen that causes gastric diseases and even gastric cancer. In this stu  ...[more]

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