Xylan deterioration approach: Purification and catalytic behavior optimization of a novel ?-1,4-d-xylanohydrolase from Geobacillus stearothermophilus KIBGE-IB29.

ABSTRACT: The ?-1,4-d-xylanohydrolase is an industry valuable catalytic protein and used to synthesize xylooligosaccharides and xylose. In the current study, ?-1,4-d-xylanohydrolase from Geobacillus stearothermophilus KIBGE-IB29 was partially purified up to 9.5-fold with a recovery yield of 52%. It exhibited optimal catalytic activity at pH-7.0 and 50?°C within 5?min. Almost 50% activity retained at pH-4.0 to 9.0 however, 70% activity observed within the range of 40?°C to 70?°C. The ?-1,4-d-xylanohydrolase showed a significant hydrolytic pattern with 48.7?kDa molecular mass. It was found that the enzymatic activity improved up to 160% with 1.0?mM ethanol. Moreover, the activity of enzyme drastically increased up to 2.3 and 1.5 fold when incubated with Tween 80 and Triton X-100 (1.0?mM), respectively. The ?-1,4-d-xylanohydrolase also retained 72% activity at -80?°C after 180 days. Such a remarkable biochemical properties of ?-1,4-d-xylanohydrolase make it possible to forecast its potential use in textile and food industries.

SUBMITTER: Bibi Z 

PROVIDER: S-EPMC6312829 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature