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Electrical Probes of DNA-Binding Proteins.

ABSTRACT: A DNA electrochemistry platform has been developed to probe proteins bound to DNA electrically. Here gold electrodes are modified with thiol-modified DNA, and DNA charge transport chemistry is used to probe DNA binding and enzymatic reaction both with redox-silent and redox-active proteins. For redox-active proteins, the electrochemistry permits the determination of redox potentials in the DNA-bound form, where comparisons to DNA-free potentials can be made using graphite electrodes without DNA modification. Importantly, electrochemistry on the DNA-modified electrodes facilitates reaction under aqueous, physiological conditions with a sensitive electrical measurement of binding and activity.

SUBMITTER: Barton JK

PROVIDER: S-EPMC6314295 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Electrical Probes of DNA-Binding Proteins.

Barton Jacqueline K JK Bartels Phillip L PL Deng Yingxin Y O'Brien Elizabeth E

Methods in enzymology 20170510

A DNA electrochemistry platform has been developed to probe proteins bound to DNA electrically. Here gold electrodes are modified with thiol-modified DNA, and DNA charge transport chemistry is used to probe DNA binding and enzymatic reaction both with redox-silent and redox-active proteins. For redox-active proteins, the electrochemistry permits the determination of redox potentials in the DNA-bound form, where comparisons to DNA-free potentials can be made using graphite electrodes without DNA ...[more]

PMID: 28645377

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