Stochasticity in transcriptional expression of a negative regulator of Arabidopsis ABA network.

ABSTRACT: Stably heritable spatiotemporal co/over-expression of distinct transcriptional regulators across generations is a desired target as they signal traffic in the cell. Here, the stability and expression pattern of AtHB7 (Arabidopsis homeodomain-leucine zipper class I) cDNA was characterized in 220 random population of transformed tomato clones where no AtHB7 orthologous has been identified in to date. Integration of p35S::AtHB7 casette was tested by the amplification of the stretches (700/425 bp) in the target by NPT II/AtHB7 oligos. Transcriptional expression pattern for the amplicons of the specific transcripts in the leaf tissues of transformants were determined by qRT-PCR. Transgene copy number was negatively correlated with transgene expression l

SUBMITTER: Pehlivan N 

PROVIDER: S-EPMC6314942 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature