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Oxidation of 5-methylaminomethyl uridine (mnm?U) by Oxone Leads to Aldonitrone Derivatives.

ABSTRACT: Oxidative RNA damage is linked to cell dysfunction and diseases. The present work focuses on the in vitro oxidation of 5-methylaminomethyl uridine (mnm?U), which belongs to the numerous post-transcriptional modifications that are found in tRNA. The reaction of oxone with mnm?U in water at pH 7.5 leads to two aldonitrone derivatives. They form by two oxidation steps and one dehydration step. Therefore, the potential oxidation products of mnm?U in vivo may not be only aldonitrones, but also hydroxylamine and imine derivatives (which may be chemically more reactive). Irradiation of aldonitrone leads to unstable oxaziridine derivatives that are susceptible to isomerization to amide or to hydrolysis to aldehyde derivative.

SUBMITTER: Zhou Q

PROVIDER: S-EPMC6315764 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Oxidation of 5-methylaminomethyl uridine (mnm⁵U) by Oxone Leads to Aldonitrone Derivatives.

Zhou Qishun Q Vu Ngoc Bao Tram BT Leszczynska Grazyna G Stigliani Jean-Luc JL Pratviel Geneviève G

Biomolecules 20181114 4

Oxidative RNA damage is linked to cell dysfunction and diseases. The present work focuses on the in vitro oxidation of 5-methylaminomethyl uridine (mnm⁵U), which belongs to the numerous post-transcriptional modifications that are found in tRNA. The reaction of oxone with mnm⁵U in water at pH 7.5 leads to two aldonitrone derivatives. They form by two oxidation steps and one dehydration step. Therefore, the potential oxidation products of mnm⁵U in vivo may not be only aldonitrones, but also hydrox ...[more]

PMID: 30441840

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