Dataset Information

Key Role of the CD56^lowCD16^low Natural Killer Cell Subset in the Recognition and Killing of Multiple Myeloma Cells.

ABSTRACT: Natural Killer (NK) cells play a pivotal role in the immunosurveillance of Multiple Myeloma (MM), but it is still undefined whether the NK cell functional properties underlying their protective activity against MM are confined to distinct NK cell populations. Interestingly, herein we report that the CD56^lowCD16^low NK cell subset displayed higher cytolytic activity compared to the other NK cell subsets (i.e., CD56^highCD16^+/-, CD56^lowCD16^high) against MM cells and its activity was impaired in MM patients. Decreased DNAM-1 expression levels were observed on the CD56^lowCD16^low NK cells during MM progression. Evaluating NK cell subset frequency after autologous hematopoietic stem cell transplantation, we found that CD56^lowCD16^low NK cells recovered earlier after transplantation. Overall, our data denote a key role of CD56^lowCD16^low subpopulation in the killing of MM cells and suggest that the reconstitution of CD56^lowCD16^low subpopulation after HSCT could be a useful approach of adoptive immunotherapy in the treatment of relapsed/refractory MM patients.

SUBMITTER: Vulpis E

PROVIDER: S-EPMC6317053 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Key Role of the CD56lowCD16low Natural Killer Cell Subset in the Recognition and Killing of Multiple Myeloma Cells.

Vulpis Elisabetta E Stabile Helena H Soriani Alessandra A Fionda Cinzia C Petrucci Maria Teresa MT Mariggio' Elena E Ricciardi Maria Rosaria MR Cippitelli Marco M Gismondi Angela A Santoni Angela A Zingoni Alessandra A

Cancers 20181129 12

Natural Killer (NK) cells play a pivotal role in the immunosurveillance of Multiple Myeloma (MM), but it is still undefined whether the NK cell functional properties underlying their protective activity against MM are confined to distinct NK cell populations. Interestingly, herein we report that the CD56lowCD16low NK cell subset displayed higher cytolytic activity compared to the other NK cell subsets (i.e., CD56highCD16+/-, CD56lowCD16 ...[more]

PMID: 30501078

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Project description:The differentiation of human induced pluripotent stem cells (hiPSCs) into T and natural killer (NK) lymphocytes opens novel possibilities for developmental studies of immune cells and in-vitro generation of cell therapy products. In particular, iPSC-derived NK cells gained interest in adoptive anti-cancer immunotherapies, since they enable generation of homogenous populations of NK cells with and without genetic engineering that can be grown at clinical scale. However, the phenotype of in-vitro generated NK cells is not well characterized. NK cells derive in the bone marrow and mature in secondary lymphoid tissues through distinct stages from CD56brightCD16- to CD56dimCD16+ NK cells that represents the most abandoned population in peripheral blood. In this study, we efficiently generated CD56+CD16+CD3- NK lymphocytes from hiPSC and characterized NK-cell development by surface expression of NK-lineage markers. Hematopoietic priming of hiPSC resulted in 31.9% to 57.4% CD34+CD45+ hematopoietic progenitor cells (HPC) that did not require enrichment for NK lymphocyte propagation. HPC were further differentiated into NK cells on OP9-DL1 feeder cells resulting in high purity of CD56brightCD16- and CD56brightCD16+ NK cells. The output of generated NK cells increased up to 40% when OP9-DL1 feeder cells were inactivated with mitomycine C. CD7 expression could be detected from the first week of differentiation indicating priming towards the lymphoid lineage. CD56brightCD16-/+ NK cells expressed high levels of DNAM-1, CD69, natural killer cell receptors NKG2A and NKG2D, and natural cytotoxicity receptors NKp46, NKp44, NKp30. Expression of NKp80 on 40% of NK cells, and a perforin+ and granzyme B+ phenotype confirmed differentiation up to stage 4b. Killer cell immunoglobulin-like receptor KIR2DL2/DL3 and KIR3DL1 were found on up to 3 and 10% of mature NK cells, respectively. NK cells were functional in terms of cytotoxicity, degranulation and antibody-dependent cell-mediated cytotoxicity.

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Key Role of the CD56^lowCD16^low Natural Killer Cell Subset in the Recognition and Killing of Multiple Myeloma Cells.

Publications

Key Role of the CD56<sup>low</sup>CD16<sup>low</sup> Natural Killer Cell Subset in the Recognition and Killing of Multiple Myeloma Cells.

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