Ontology highlight
ABSTRACT:
SUBMITTER: Moquin DM
PROVIDER: S-EPMC6317314 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Moquin David M DM Genois Marie-Michelle MM Zhang Jia-Min JM Ouyang Jian J Yadav Tribhuwan T Buisson Rémi R Yazinski Stephanie A SA Tan Jun J Boukhali Myriam M Gagné Jean-Philippe JP Poirier Guy G GG Lan Li L Haas Wilhelm W Zou Lee L
Genes & development 20181219 1-2
Numerous DNA repair and signaling proteins function at DNA damage sites to protect the genome. Here, we show that fusion of the promiscuous biotin ligase BirA<sup>R118G</sup> with RAD18 leads to localized protein biotinylation at DNA damage sites, allowing identification of ZPET (zinc finger protein proximal to RAD eighteen)/ZNF280C as a potential DNA damage response (DDR) protein. ZPET binds ssDNA and localizes to DNA double-strand breaks (DSBs) and stalled replication forks. In vitro, ZPET inh ...[more]