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Spotiton: New features and applications.

ABSTRACT: We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ?3?Å resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples.

SUBMITTER: Dandey VP

PROVIDER: S-EPMC6317895 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Spotiton: New features and applications.

Dandey Venkata P VP Wei Hui H Zhang Zhening Z Tan Yong Zi YZ Acharya Priyamvada P Eng Edward T ET Rice William J WJ Kahn Peter A PA Potter Clinton S CS Carragher Bridget B

Journal of structural biology 20180501 2

We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ∼3 Å resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples. ...[more]

PMID: 29366716

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