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Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry.

ABSTRACT: The ability to design detergents that are suitable for protein analysis by mass spectrometry (MS) represents an on-going challenge in the field of native MS. Desirable detergent characteristics include charge-reducing properties and low gas-phase stabilities of complexes formed with proteins. In this work, the gas-phase properties of oligoglycerol detergents (OGDs) are optimized by fine tuning of their molecular structure. Furthermore, a tandem mass spectrometry (MS/MS) approach is presented that estimates the gas-phase properties of detergents simply by studying the dissociation behaviour of protein-detergent complexes (PDCs) formed with the soluble protein ?-lactoglobulin (BLG). Graphical Abstract ?.

SUBMITTER: Urner LH

PROVIDER: S-EPMC6318253 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry.

Urner Leonhard H LH Maier Yasmine B YB Haag Rainer R Pagel Kevin K

Journal of the American Society for Mass Spectrometry 20181001 1

The ability to design detergents that are suitable for protein analysis by mass spectrometry (MS) represents an on-going challenge in the field of native MS. Desirable detergent characteristics include charge-reducing properties and low gas-phase stabilities of complexes formed with proteins. In this work, the gas-phase properties of oligoglycerol detergents (OGDs) are optimized by fine tuning of their molecular structure. Furthermore, a tandem mass spectrometry (MS/MS) approach is presented tha ...[more]

PMID: 30276626

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