Dataset Information

Genome Sequence of Bacillus velezensis 5RB, an Overproducer of 2,3-Butanediol.

ABSTRACT: Bacillus velezensis 5RB is capable of producing large amounts of 2,3-butanediol. Whole-genome sequencing revealed that the strain contains one circular chromosome of 3.91 Mbp without plasmids. A large part of the genome is devoted to carbohydrate metabolism, encoding an abundance of enzymes participating in polysaccharide utilization pathways.

SUBMITTER: Petrova P

PROVIDER: S-EPMC6318372 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Genome Sequence of Bacillus velezensis 5RB, an Overproducer of 2,3-Butanediol.

Petrova Penka P Velikova Petya P Petrov Kaloyan K

Microbiology resource announcements 20190103 1

Bacillus velezensis 5RB is capable of producing large amounts of 2,3-butanediol. Whole-genome sequencing revealed that the strain contains one circular chromosome of 3.91 Mbp without plasmids. A large part of the genome is devoted to carbohydrate metabolism, encoding an abundance of enzymes participating in polysaccharide utilization pathways. ...[more]

PMID: 30637401

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Project description:BackgroundPreviously, a safe strain, Bacillus amyloliquefaciens B10-127 was identified as an excellent candidate for industrial-scale microbial fermentation of 2,3-butanediol (2,3-BD). However, B. amyloliquefaciens fermentation yields large quantities of acetoin, lactate and succinate as by-products, and the 2,3-BD yield remains prohibitively low for commercial production.Methodology/principal findingsIn the 2,3-butanediol metabolic pathway, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of 3-phosphate glyceraldehyde to 1,3-bisphosphoglycerate, with concomitant reduction of NAD(+) to NADH. In the same pathway, 2,3-BD dehydrogenase (BDH) catalyzes the conversion of acetoin to 2,3-BD with concomitant oxidation of NADH to NAD(+). In this study, to improve 2,3-BD production, we first over-produced NAD(+)-dependent GAPDH and NADH-dependent BDH in B. amyloliquefaciens. Excess GAPDH reduced the fermentation time, increased the 2,3-BD yield by 12.7%, and decreased the acetoin titer by 44.3%. However, the process also enhanced lactate and succinate production. Excess BDH increased the 2,3-BD yield by 16.6% while decreasing acetoin, lactate and succinate production, but prolonged the fermentation time. When BDH and GAPDH were co-overproduced in B. amyloliquefaciens, the fermentation time was reduced. Furthermore, in the NADH-dependent pathways, the molar yield of 2,3-BD was increased by 22.7%, while those of acetoin, lactate and succinate were reduced by 80.8%, 33.3% and 39.5%, relative to the parent strain. In fed-batch fermentations, the 2,3-BD concentration was maximized at 132.9 g/l after 45 h, with a productivity of 2.95 g/l·h.Conclusions/significanceCo-overexpression of bdh and gapA genes proved an effective method for enhancing 2,3-BD production and inhibiting the accumulation of unwanted by-products (acetoin, lactate and succinate). To our knowledge, we have attained the highest 2,3-BD fermentation yield thus far reported for safe microorganisms.

Dataset Information

Genome Sequence of Bacillus velezensis 5RB, an Overproducer of 2,3-Butanediol.

Publications

Genome Sequence of Bacillus velezensis 5RB, an Overproducer of 2,3-Butanediol.

Similar Datasets

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