Next Generation-Targeted Amplicon Sequencing (NG-TAS): an optimised protocol and computational pipeline for cost-effective profiling of circulating tumour DNA.
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ABSTRACT:
SUBMITTER: Gao M
PROVIDER: S-EPMC6320579 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Publications
Next Generation-Targeted Amplicon Sequencing (NG-TAS): an optimised protocol and computational pipeline for cost-effective profiling of circulating tumour DNA.
Gao Meiling M Callari Maurizio M Beddowes Emma E Sammut Stephen-John SJ Grzelak Marta M Biggs Heather H Jones Linda L Boumertit Abdelhamid A Linn Sabine C SC Cortes Javier J Oliveira Mafalda M Baird Richard R Chin Suet-Feung SF Caldas Carlos C
Genome medicine 20190104 1
Circulating tumour DNA (ctDNA) detection and monitoring have enormous potential clinical utility in oncology. We describe here a fast, flexible and cost-effective method to profile multiple genes simultaneously in low input cell-free DNA (cfDNA): Next Generation-Targeted Amplicon Sequencing (NG-TAS). We designed a panel of 377 amplicons spanning 20 cancer genes and tested the NG-TAS pipeline using cell-free DNA from two HapMap lymphoblastoid cell lines. NG-TAS consistently detected mutations in ...[more]
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