Dataset Information

Genome-Wide Identification and Analysis of HAK/KUP/KT Potassium Transporters Gene Family in Wheat (Triticum aestivum L.).

ABSTRACT: In plants, the HAK (high-affinity K⁺)/KUP (K⁺ uptake)/KT (K⁺ transporter) family represents a large group of potassium transporters that play important roles in plant growth and environmental adaptation. Although HAK/KUP/KT genes have been extensively investigated in many plant species, they remain uncharacterized in wheat, especially those involved in the response to environmental stresses. In this study, 56 wheat HAK/KUP/KT (hereafter called TaHAKs) genes were identified by a genome-wide search using recently released wheat genomic data. Phylogenetic analysis grouped these genes into four clusters (Ι, II, III, IV), containing 22, 19, 7 and 8 genes, respectively. Chromosomal distribution, gene structure, and conserved motif analyses of the 56 TaHAK genes were subsequently performed. In silico RNA-seq data analysis revealed that TaHAKs from clusters II and III are constitutively expressed in various wheat tissues, while most genes from clusters I and IV have very low expression levels in the examined tissues at different developmental stages. qRT-PCR analysis showed that expression levels of TaHAK genes in wheat seedlings were significantly up- or downregulated when seedlings were exposed to K⁺ deficiency, high salinity, or dehydration. Furthermore, we functionally characterized TaHAK1b-2BL and showed that it facilitates K⁺ transport in yeast. Collectively, these results provide valuable information for further functional studies of TaHAKs, and contribute to a better understanding of the molecular basis of wheat development and stress tolerance.

SUBMITTER: Cheng X

PROVIDER: S-EPMC6321448 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Genome-Wide Identification and Analysis of HAK/KUP/KT Potassium Transporters Gene Family in Wheat (Triticum aestivum L.).

Cheng Xiyong X Liu Xiaodan X Mao Weiwei W Zhang Xurui X Chen Shulin S Zhan Kehui K Bi Huihui H Xu Haixia H

International journal of molecular sciences 20181210 12

In plants, the HAK (high-affinity K⁺)/KUP (K⁺ uptake)/KT (K⁺ transporter) family represents a large group of potassium transporters that play important roles in plant growth and environmental adaptation. Although HAK/KUP/KT genes have been extensively investigated in many plant species, they remain uncharacterized in wheat, especially those involved in the response to environmental stresses. In this study, 56 wheat HAK/KUP/KT (hereafter called TaHAKs) genes were identified b ...[more]

PMID: 30544665

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Project description:The HAK/KUP/KT (High-affinity K+ transporters/K+ uptake permeases/K+ transporters) is the largest and most dominant potassium transporter family in plants, playing a crucial role in various biological processes. However, our understanding of HAK/KUP/KT gene family in potato (Solanum tuberosum L.) remains limited and unclear. In this study, 24 HAK/KUP/KT genes (StHAKs) were identified through a genome-wide analysis and were found to be unevenly distributed across ten chromosomes. Based on phylogenetic analysis, these StHAK gene family members were classified into four distinct clusters. All StHAK protein sequences contained the conserved motifs and domains. Promoter cis-acting elements analysis revealed that most StHAK gene family members in potatoes were associated with responses to light and hormones such as abscisic acid or methyl jasmonate, however, many motifs responsive to hormones and stress conditions have not been clearly studied or reported in plants. Synteny analysis suggested that 33, 19, 8, 1 StHAK genes were orthologous to those in soybean, cassava, Arabidopsis and rice, respectively. The previously published RNA-seq results, transcriptomic data and qRT-PCR experiments indicated that the expression profiles of these StHAK genes were tissue-specific and were influenced by multiple factors, including biotic and abiotic stress, hormone, potassium fertilizer. To provide a clear and convenient view of StHAK gene expression across different tissues in potato, we generated a cartoon heatmap to vividly illustrate the tissue-specific expression of StHAK genes, which is unprecedented in the gene family analysis of potato. At last, we identified genes such as StHAK8, StHAK14, and StHAK22 with high expression in potato tubers using qRT-PCR, suggesting their potential involvement in tuber growth and development. This can contribute to a deeper understanding of the mechanism of potassium absorption and transportation in potatoes. It has laid a solid theoretical foundation for the genetic regulation of potassium nutritional efficiency in potatoes and the breeding of potato varieties with high potassium efficiency.

Project description:BackgroundTea plant is one of the most important non-alcoholic beverage crops worldwide. While potassium (K+) is an essential macronutrient and greatly affects the growth and development of plants, the molecular mechanism underlying K+ uptake and transport in tea plant root, especially under limited-K+ conditions, is still poorly understood. In plants, HAK/KUP/KT family members play a crucial role in K+ acquisition and translocation, growth and development, and response to stresses. Nevertheless, the biological functions of these genes in tea plant are still in mystery, especially their roles in K+ uptake and stress responses.ResultsIn this study, a total of 21 non-redundant HAK/KUP/KT genes (designated as CsHAKs) were identified in tea plant. Phylogenetic and structural analysis classified the CsHAKs into four clusters (I, II, III, IV), containing 4, 8, 4 and 5 genes, respectively. Three major categories of cis-acting elements were found in the promoter regions of CsHAKs. Tissue-specific expression analysis indicated extremely low expression levels in various tissues of cluster I CsHAKs with the exception of a high root expression of CsHAK4 and CsHAK5, a constitutive expression of clusters II and III CsHAKs, and a moderate cluster IV CsHAKs expression. Remarkably, the transcript levels of CsHAKs in roots were significantly induced or suppressed after exposure to K+ deficiency, salt and drought stresses, and phytohormones treatments. Also notably, CsHAK7 was highly expressed in all tissues and was further induced under various stress conditions. Therefore, functional characterization of CsHAK7 was performed, and the results demostrated that CsHAK7 locates on plasma membrane and plays a key role in K+ transport in yeast. Taken together, the results provide promising candidate CsHAKs for further functional studies and contribute to the molecular breeding for new tea plants varieties with highly efficient utilization of K+.ConclusionThis study demonstrated the first genome-wide analysis of CsHAK family genes of tea plant and provides a foundation for understanding the classification and functions of the CsHAKs in tea plants.

Dataset Information

Genome-Wide Identification and Analysis of HAK/KUP/KT Potassium Transporters Gene Family in Wheat (Triticum aestivum L.).

Publications

Genome-Wide Identification and Analysis of HAK/KUP/KT Potassium Transporters Gene Family in Wheat (<i>Triticum aestivum</i> L.).

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