Altered composition and phenotype of mucosal-associated invariant T cells in early untreated rheumatoid arthritis.
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ABSTRACT: Background
Mucosal-associated invariant T (MAIT) cells are innate-like T cells that recognise bacterial metabolites presented by MHC class I-related protein 1 (MR1). Bacterial dysbiosis has been implicated in auto-inflammatory disease development. We investigated MAIT cells in early, untreated rheumatoid arthritis (RA) and spondyloarthritis (SpA) patients.Methods
Blood and synovial fluid mononuclear cells obtained from patients (SpA/RA) and controls were stimulated with fixed Escherichia coli to provide MAIT ligand. Cells were analysed by flow cytometry and MAIT cells were identified by MR1-5-OP-RU tetramers. Synovial biopsies were studied by confocal microscopy.Results
Peripheral and synovial CD3+ MR1-tet+ MAIT cell frequencies were comparable in all groups. MAIT cells were detected in RA and SpA synovium based on CD3, CD161 and V?7.2 expression. Peripheral RA MAIT cells were mostly CD4+ (controls 8.3%, SpA 12.3%, RA 52.6%; p?ConclusionIn early untreated RA patients, the peripheral MAIT cell composition was altered, with reduced levels of CD161 expression, and cells were hyporesponsive to stimulation. MAIT cell dysfunction may provide a link between the microbiome and development of RA.
SUBMITTER: Koppejan H
PROVIDER: S-EPMC6321723 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Publications
Altered composition and phenotype of mucosal-associated invariant T cells in early untreated rheumatoid arthritis.
Koppejan Hester H Jansen Diahann T S L DTSL Hameetman Marjolijn M Thomas Ranjeny R Toes Rene E M REM van Gaalen Floris A FA
Arthritis research & therapy 20190105 1
<h4>Background</h4>Mucosal-associated invariant T (MAIT) cells are innate-like T cells that recognise bacterial metabolites presented by MHC class I-related protein 1 (MR1). Bacterial dysbiosis has been implicated in auto-inflammatory disease development. We investigated MAIT cells in early, untreated rheumatoid arthritis (RA) and spondyloarthritis (SpA) patients.<h4>Methods</h4>Blood and synovial fluid mononuclear cells obtained from patients (SpA/RA) and controls were stimulated with fixed Esc ...[more]
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