Morphokinetic Evaluation of Embryo Development in a Mouse Model: Functional and Molecular Correlates.

ABSTRACT: Although time-lapse analysis of early embryo cleavage parameters (morphokinetics) predicts blastocyst development, it has not been definitively linked to establishing pregnancy and live birth. For example, a direct comparison of the developmental potential of embryos with optimal kinetic parameters compared to suboptimal kinetics has not been performed with human embryos. To ascertain whether such a linkage exists, we developed a mouse model of morphokinetic analysis of early embryo cleavage using time-lapse microscopy to predict blastocyst formation and tested whether cleavage parameters predict pregnancy outcome by transferring morphokinetically optimal and suboptimal embryos into a single host. Using classification and regression trees, we established that the timing of the second and t

SUBMITTER: Weinerman R 

PROVIDER: S-EPMC6322449 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature