Comparison of Bacillus subtilis transcriptome profiles from two separate missions to the International Space Station.

ABSTRACT: The human spaceflight environment is notable for the unique factor of microgravity, which exerts numerous physiologic effects on macroscopic organisms, but how this environment may affect single-celled microbes is less clear. In an effort to understand how the microbial transcriptome responds to the unique environment of spaceflight, the model Gram-positive bacterium Bacillus subtilis was flown on two separate missions to the International Space Station in experiments dubbed BRIC-21 and BRIC-23. Cells were grown to late-exponential/early stationary phase, frozen, then returned to Earth for RNA-seq analysis in parallel with matched ground control samples. A total of 91 genes were significantly differentially expressed in both experiments; 55 exhibiting higher transcript levels in fli

SUBMITTER: Morrison MD 

PROVIDER: S-EPMC6323116 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature