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Spatial dynamics of chromosome translocations in living cells.

ABSTRACT: Chromosome translocations are a hallmark of cancer cells. We have developed an experimental system to visualize the formation of translocations in living cells and apply it to characterize the spatial and dynamic properties of translocation formation. We demonstrate that translocations form within hours of the occurrence of double-strand breaks (DSBs) and that their formation is cell cycle-independent. Translocations form preferentially between prepositioned genome elements, and perturbation of key factors of the DNA repair machinery uncouples DSB pairing from translocation formation. These observations generate a spatiotemporal framework for the formation of translocations in living cells.

SUBMITTER: Roukos V

PROVIDER: S-EPMC6324928 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Spatial dynamics of chromosome translocations in living cells.

Roukos Vassilis V Voss Ty C TC Schmidt Christine K CK Lee Seungtaek S Wangsa Darawalee D Misteli Tom T

Science (New York, N.Y.) 20130801 6146

Chromosome translocations are a hallmark of cancer cells. We have developed an experimental system to visualize the formation of translocations in living cells and apply it to characterize the spatial and dynamic properties of translocation formation. We demonstrate that translocations form within hours of the occurrence of double-strand breaks (DSBs) and that their formation is cell cycle-independent. Translocations form preferentially between prepositioned genome elements, and perturbation of ...[more]

PMID: 23929981

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