Ontology highlight
ABSTRACT:
SUBMITTER: Nasri M
PROVIDER: S-EPMC6325296 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Blood advances 20190101 1
CRISPR/Cas9-mediated gene editing of stem cells and primary cell types has several limitations for clinical applications. The direct delivery of ribonucleoprotein (RNP) complexes consisting of Cas9 nuclease and guide RNA (gRNA) has improved DNA- and virus-free gene modifications, but it does not enable the essential enrichment of the gene-edited cells. Here, we established a protocol for the fluorescent labeling and delivery of CRISPR/Cas9-gRNA RNP in primary human hematopoietic stem and progeni ...[more]