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Coprs inactivation leads to a derepression of LINE1 transposons in spermatocytes.

ABSTRACT: Repression of retrotransposons is essential for genome integrity during germ cell development and is tightly controlled through epigenetic mechanisms. In primordial germ cells, protein arginine N-methyltransferase (Prmt5) is involved in retrotransposon repression by methylating Piwi proteins, which is part of the piRNA pathway. Here, we show that in mice, genetic inactivation of coprs (which is highly expressed in testis and encodes a histone-binding protein required for the targeting of Prmt5 activity) affects the maturation of spermatogonia to spermatids. Mass spectrometry analysis revealed the presence of Miwi in testis protein lysates immunoprecipitated with an anti-Coprs antibody. The observed deregulation of Miwi and pachytene pre-piRNAs levels and the derepression of LINE1 repetitive sequences observed in coprs-/- mice suggest that Coprs is implicated in genome surveillance mechanisms.

SUBMITTER: Paul C

PROVIDER: S-EPMC6325579 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Coprs inactivation leads to a derepression of LINE1 transposons in spermatocytes.

Paul Conception C Delpech Hélène H Haouzi Delphine D Hamamah Samir S Sardet Claude C Fabbrizio Eric E

FEBS open bio 20181219 1

Repression of retrotransposons is essential for genome integrity during germ cell development and is tightly controlled through epigenetic mechanisms. In primordial germ cells, protein arginine N-methyltransferase (Prmt5) is involved in retrotransposon repression by methylating Piwi proteins, which is part of the piRNA pathway. Here, we show that in mice, genetic inactivation of coprs (which is highly expressed in testis and encodes a histone-binding protein required for the target ...[more]

PMID: 30652083

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