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Lipogels for Encapsulation of Hydrophilic Proteins and Hydrophobic Small Molecules.

ABSTRACT: Lipid-polymer hybrid materials have the potential to exhibit enhanced stability and loading capabilities in comparison to parent liposome or polymer materials. However, complexities lie in formulating and characterizing such complex nanomaterials. Here we describe a lipid-coated polymer gel (lipogel) formulated using a single-pot methodology, where self-assembling liposomes template a UV-curable polymer gel core. Using fluorescently labeled lipids, protein, and hydrophobic molecules, we characterized their formation, purification, stability, and encapsulation efficiency via common instrumentation methods such as dynamic light scattering (DLS), matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS), UV-vis spectroscopy, fluorescence spectroscopy, and single-particle total internal reflection fluorescence (TIRF) microscopy. In addition, we confirmed that these dual-guest-loaded lipogels are stable in solution for several months. The simplicity of this complete aqueous formation and noncovalent dual-guest encapsulation holds potential as a tunable nanomaterial scaffold.

SUBMITTER: Homyak CC 

PROVIDER: S-EPMC6326177 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Lipogels for Encapsulation of Hydrophilic Proteins and Hydrophobic Small Molecules.

Homyak Celia C CC   Fernandez Ann A   Touve Mollie A MA   Zhao Bo B   Anson Francesca F   Hardy Jeanne A JA   Vachet Richard W RW   Gianneschi Nathan C NC   Ross Jennifer L JL   Thayumanavan S S  

Biomacromolecules 20171215 1

Lipid-polymer hybrid materials have the potential to exhibit enhanced stability and loading capabilities in comparison to parent liposome or polymer materials. However, complexities lie in formulating and characterizing such complex nanomaterials. Here we describe a lipid-coated polymer gel (lipogel) formulated using a single-pot methodology, where self-assembling liposomes template a UV-curable polymer gel core. Using fluorescently labeled lipids, protein, and hydrophobic molecules, we characte  ...[more]

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