Gene expression profiles of the meniscus avascular phenotype: A guide for meniscus tissue engineering.
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ABSTRACT:
SUBMITTER: Grogan SP
PROVIDER: S-EPMC6326361 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Publications
Gene expression profiles of the meniscus avascular phenotype: A guide for meniscus tissue engineering.
Grogan Shawn P SP Duffy Stuart F SF Pauli Chantal C Lotz Martin K MK D'Lima Darryl D DD
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 20180314 7
Avascular (Avas) meniscus regeneration remains a challenge, which is partly a consequence of our limited knowledge of the cells that maintain this tissue region. In this study, we utilized microarrays to characterize gene expression profiles of intact human Avas meniscus tissue and of cells following culture expansion. Using these data, we examined various 3D culture conditions to redifferentiate Avas cells toward the tissue phenotype. RNA was isolated from either the tissue directly or followin ...[more]
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