Ontology highlight
ABSTRACT:
SUBMITTER: Amundsen SK
PROVIDER: S-EPMC6326792 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Amundsen Susan K SK Smith Gerald R GR
Nucleic acids research 20190101 1
In bacteria, repair of DNA double-strand breaks uses a highly conserved helicase-nuclease complex to unwind DNA from a broken end and cut it at specific DNA sequences called Chi. In Escherichia coli the RecBCD enzyme also loads the DNA strand-exchange protein RecA onto the newly formed end, resulting in a recombination hotspot at Chi. Chi hotspots regulate multiple RecBCD activities by altering RecBCD's conformation, which is proposed to include the swinging of the RecB nuclease domain on the 19 ...[more]