Ontology highlight
ABSTRACT:
SUBMITTER: Matoba S
PROVIDER: S-EPMC6326833 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Matoba Shogo S Wang Huihan H Jiang Lan L Lu Falong F Iwabuchi Kumiko A KA Wu Xiaoji X Inoue Kimiko K Yang Lin L Press William W Lee Jeannie T JT Ogura Atsuo A Shen Li L Zhang Yi Y
Cell stem cell 20180719 3
Animal cloning can be achieved through somatic cell nuclear transfer (SCNT), although the live birth rate is relatively low. Recent studies have identified H3K9me3 in donor cells and abnormal Xist activation as epigenetic barriers that impede SCNT. Here we overcome these barriers using a combination of Xist knockout donor cells and overexpression of Kdm4 to achieve more than 20% efficiency of mouse SCNT. However, post-implantation defects and abnormal placentas were still observed, indicating th ...[more]