Dataset Information

HIV-associated sensory polyneuropathy and neuronal injury are associated with miRNA-455-3p induction.

ABSTRACT: Symptomatic distal sensory polyneuropathy (sDSP) is common and debilitating in people with HIV/AIDS, leading to neuropathic pain, although the condition's cause is unknown. To investigate biomarkers and associated pathogenic mechanisms for sDSP, we examined plasma miRNA profiles in HIV/AIDS patients with sDSP or without sDSP in 2 independent cohorts together with assessing related pathogenic effects. Several miRNAs were found to be increased in the Discovery Cohort (sDSP, n = 29; non-DSP, n = 40) by array analyses and were increased in patients with sDSP compared with patients without sDSP. miR-455-3p displayed a 12-fold median increase in the sDSP group, which was confirmed by machine learning analyses and verified by reverse transcription PCR. In the Validation Cohort (sDSP n = 16, non-DSP n = 20, healthy controls n = 15), significant upregulation of miR-455-3p was also observed in the sDSP group. Bioinformatics revealed that miR-455-3p targeted multiple host genes implicated in peripheral nerve maintenance, including nerve growth factor (NGF) and related genes. Transfection of cultured human dorsal root ganglia with miR-455-3p showed a concentration-dependent reduction in neuronal ?-III tubulin expression. Human neurons transfected with miR-455-3p demonstrated reduced neurite outgrowth and NGF expression that was reversed by anti-miR-455-3p antagomir cotreatment. miR-455-3p represents a potential biomarker for HIV-associated sDSP and might also exert pathogenic effects leading to sDSP.

SUBMITTER: Asahchop EL

PROVIDER: S-EPMC6328026 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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HIV-associated sensory polyneuropathy and neuronal injury are associated with miRNA-455-3p induction.

Asahchop Eugene L EL Branton William G WG Krishnan Anand A Chen Patricia A PA Yang Dong D Kong Linglong L Zochodne Douglas W DW Brew Bruce J BJ Gill M John MJ Power Christopher C

JCI insight 20181206 23

Symptomatic distal sensory polyneuropathy (sDSP) is common and debilitating in people with HIV/AIDS, leading to neuropathic pain, although the condition's cause is unknown. To investigate biomarkers and associated pathogenic mechanisms for sDSP, we examined plasma miRNA profiles in HIV/AIDS patients with sDSP or without sDSP in 2 independent cohorts together with assessing related pathogenic effects. Several miRNAs were found to be increased in the Discovery Cohort (sDSP, n = 29; non-DSP, n = 40 ...[more]

PMID: 30518697

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Dataset Information

HIV-associated sensory polyneuropathy and neuronal injury are associated with miRNA-455-3p induction.

Publications

HIV-associated sensory polyneuropathy and neuronal injury are associated with miRNA-455-3p induction.

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