Ontology highlight
ABSTRACT:
SUBMITTER: Marinho RC
PROVIDER: S-EPMC6328810 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Marinho Rebeca C RC Martins Gabrielle R GR Souza Kelma C KC Sousa Ana Lídia M ALM Silva Sabrina Tainah C STC Nobre Juliana A JA Teixeira Maria F S MFS
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20180813
Small ruminant lentiviruses (SRLV) have high genetic variability which results in different viral strains around the world. This create a challenge to design sensible primers for molecular diagnosis in different regions. This work proposes a protocol of duplex nested-PCR for the precise diagnosis of SRLV. The technique was designed and tested with the control strains CAEV Co and MVV 1514. Then, field strains were submitted to the same protocol of duplex nested-PCR. Blood samples of sheep and goa ...[more]