Dataset Information

Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content.

ABSTRACT: BACKGROUND:Rapeseed (Brassica napus, B. napus) is an important oil seed crop in the world. Previous studies showed that seed germination vigor might be correlated with seed oil content in B. napus, but the regulation mechanism for seed germination has not yet been explained clearly. Dissecting the regulation mechanism of seed germination and germination vigor is necessary. RESULTS:Here, proteomic and genomic approaches were used to analyze the germination process in B. napus seeds with different oil content. The identification of 165 differentially expressed proteins (DEPs) in the germinating seeds of B. napus with high and low oil content was accomplished by two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE). The comparative proteomic results revealed that seeds with high oil content had higher metabolic activity, especially for sulfur amino acid metabolism. Thirty-one unique genes were shown to be significantly changed during germination between the seeds with high and low oil content, and thirteen of these genes were located within the confidence interval of germination-related quantitative trait locus (QTLs), which might play an important role in regulating seed germination vigor. CONCLUSIONS:The present results are of importance for the understanding of the regulation mechanism for seed germination vigor in B. napus.

SUBMITTER: Gu J

PROVIDER: S-EPMC6329107 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content.

Gu Jianwei J Hou Dalin D Li Yonghong Y Chao Hongbo H Zhang Kai K Wang Hao H Xiang Jun J Raboanatahiry Nadia N Wang Baoshan B Li Maoteng M

BMC plant biology 20190111 1

<h4>Background</h4>Rapeseed (Brassica napus, B. napus) is an important oil seed crop in the world. Previous studies showed that seed germination vigor might be correlated with seed oil content in B. napus, but the regulation mechanism for seed germination has not yet been explained clearly. Dissecting the regulation mechanism of seed germination and germination vigor is necessary.<h4>Results</h4>Here, proteomic and genomic approaches were used to analyze the germination process in B. napus seeds ...[more]

PMID: 30634904

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Project description:Seed germination is a complex trait determined by the interaction of hormonal, metabolic, genetic, and environmental components. Variability of this trait in crops has a big impact on seedling establishment and yield in the field. Classical studies of this trait in crops have focused mainly on the analyses of one level of regulation in the cascade of events leading to seed germination. We have carried out an integrative and extensive approach to deepen our understanding of seed germination in Brassica napus by generating transcriptomic, metabolic, and hormonal data at different stages upon seed imbibition. Deep phenotyping of different seed germination-associated traits in six winter-type B. napus accessions has revealed that seed germination kinetics, in particular seed germination speed, are major contributors to the variability of this trait. Metabolic profiling of these accessions has allowed us to describe a common pattern of metabolic change and to identify the levels of malate and aspartate metabolites as putative metabolic markers to estimate germination performance. Additionally, analysis of seed content of different hormones suggests that hormonal balance between ABA, GA, and IAA at crucial time points during this process might underlie seed germination differences in these accessions. In this study, we have also defined the major transcriptome changes accompanying the germination process in B. napus. Furthermore, we have observed that earlier activation of key germination regulatory genes seems to generate the differences in germination speed observed between accessions in B. napus. Finally, we have found that protein-protein interactions between some of these key regulator are conserved in B. napus, suggesting a shared regulatory network with other plant species. Altogether, our results provide a comprehensive and detailed picture of seed germination dynamics in oilseed rape. This new framework will be extremely valuable not only to evaluate germination performance of B. napus accessions but also to identify key targets for crop improvement in this important process.

Dataset Information

Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content.

Publications

Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content.

Similar Datasets

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