Dataset Information

Effect of the lipoxygenase inhibitor baicalein on bone tissue and bone healing in ovariectomized rats.

ABSTRACT:

Background

Osteoporosis is one of the world's major medical burdens in the twenty-first century. Pharmaceutical intervention currently focusses on decelerating bone loss, but phytochemicals such as baicalein, which is a lipoxygenase inhibitor, may rescue bone loss. Studies evaluating the effect of baicalein in vivo are rare.

Methods

We administered baicalein to sixty-one three-month-old female Sprague-Dawley rats. They were divided into five groups, four of which were ovariectomized (OVX) and one non-ovariectomized (NON-OVX). Eight weeks after ovariectomy, bilateral tibial osteotomy with plate osteosynthesis was performed and bone formation quantified. Baicalein was administered subcutaneously using three doses (C1: 1?mg/kg BW; C2: 10?mg/kg BW; and C3: 100?mg/kg BW) eight weeks after ovariectomy for four weeks. Finally, femora and tibiae were collected. Biomechanical tests, micro-CT, ashing, histological and gene expression analyses were performed.

Results

Biomechanical properties were unchanged in tibiae and reduced in femora. In tibiae, C1 treatment enhanced callus density and cortical width and decreased callus area. In the C3 group, callus formation was reduced during the first 3?weeks after osteotomy, correlating to a higher mRNA expression of Osteocalcin, Tartrate-resistant acid phosphatase and Rankl. In femora, baicalein treatments did not alter bone parameters.

Conclusions

Baicalein enhanced callus density and cortical width but impaired early callus formation in tibiae. In femora, it diminished the biomechanical properties and calcium-to-phosphate ratio. Thus, it is not advisable to apply baicalein to treat early bone fractures. To determine the exact effects on bone healing, further studies in which baicalein treatments are started at different stages of healing are needed.

SUBMITTER: Saul D

PROVIDER: S-EPMC6329162 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Effect of the lipoxygenase inhibitor baicalein on bone tissue and bone healing in ovariectomized rats.

Saul Dominik D Weber Marie M Zimmermann Marc Hendrik MH Kosinsky Robyn Laura RL Hoffmann Daniel Bernd DB Menger Björn B Taudien Stefan S Lehmann Wolfgang W Komrakova Marina M Sehmisch Stephan S

Nutrition & metabolism 20190111

<h4>Background</h4>Osteoporosis is one of the world's major medical burdens in the twenty-first century. Pharmaceutical intervention currently focusses on decelerating bone loss, but phytochemicals such as baicalein, which is a lipoxygenase inhibitor, may rescue bone loss. Studies evaluating the effect of baicalein in vivo are rare.<h4>Methods</h4>We administered baicalein to sixty-one three-month-old female Sprague-Dawley rats. They were divided into five groups, four of which were ovariectomiz ...[more]

PMID: 30651746

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Dataset Information

Effect of the lipoxygenase inhibitor baicalein on bone tissue and bone healing in ovariectomized rats.

Background

Methods

Results

Conclusions

Publications

Effect of the lipoxygenase inhibitor baicalein on bone tissue and bone healing in ovariectomized rats.

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