Unknown

Dataset Information

0

MiR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA.


ABSTRACT:

Background

miR-302 cluster has been reported as a tumor suppressor in many human cancers; yet, its function in chronic myeloid leukemia (CML) tumorigenesis remains largely unclear. The study was aimed to explore the functional roles of miR-302 cluster in CML progression.

Materials and methods

Quantitative reverse transcriptase PCR and Western blot were performed to evaluate miR-302 cluster and vascular endothelial growth factor A (VEGFA) expression levels. Cell Counting Kit-8 assay, colony formation assay and human umbilical vein endothelial cell line capillary tube formation were used to determine the influence of miR-302 cluster on the growth and angiogenesis of K562 cells, respectively. Luciferase reporter assay was employed to confirm the direct target interaction between miR-302 cluster and VEGFA.

Results

This study demonstrated that miR-302 cluster was frequently downregulated in CML samples and cell lines and high level of miR-302 cluster was significantly associated with good prognosis of CML patients. Compared with miRNA negative control, miR-302 cluster mimics obviously suppressed cell growth, colony formation and angiogenesis. Further studies revealed that VEGFA was a direct target gene of miR-302 cluster. Moreover, overexpression of VEGFA dramatically abated the inhibition of miR-302 cluster on cell growth and angiogenesis.

Conclusion

The present study, for the first time, identified miR-302 cluster as a tumor suppressor, and overexpression of miR-302 cluster inhibited growth and angiogenesis in K562 cells. miR-302 cluster may be a potential therapeutic target in CML to develop the adjuvant antiangiogenic therapy based on VEGFA.

SUBMITTER: Cao J 

PROVIDER: S-EPMC6329480 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

altmetric image

Publications

miR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA.

Cao Jiang J   Li Li L   Han Xiao X   Cheng Hai H   Chen Wei W   Qi Kunming K   Chen Chong C   Wu Qingyun Q   Niu Mingshan M   Zeng Lingyu L   Xu Kailin K  

OncoTargets and therapy 20190108


<h4>Background</h4>miR-302 cluster has been reported as a tumor suppressor in many human cancers; yet, its function in chronic myeloid leukemia (CML) tumorigenesis remains largely unclear. The study was aimed to explore the functional roles of miR-302 cluster in CML progression.<h4>Materials and methods</h4>Quantitative reverse transcriptase PCR and Western blot were performed to evaluate miR-302 cluster and vascular endothelial growth factor A (VEGFA) expression levels. Cell Counting Kit-8 assa  ...[more]

Similar Datasets

| S-EPMC6372610 | biostudies-literature
| S-EPMC5041945 | biostudies-other
| S-EPMC3644734 | biostudies-literature
| S-EPMC7295066 | biostudies-literature
| S-EPMC8661028 | biostudies-literature
| S-EPMC7214437 | biostudies-literature
| S-EPMC7373746 | biostudies-literature
| S-EPMC8144938 | biostudies-literature
| S-EPMC4015315 | biostudies-literature
| S-EPMC4691035 | biostudies-literature