Dataset Information

Circulating miR-1290 and miR-320d as Novel Diagnostic Biomarkers of Human Colorectal Cancer.

ABSTRACT: Background: The lack of screening methods with high diagnostic utility leads to colorectal cancer (CRC) patients usually diagnosed in advanced stages which results in high mortality. This study aimed to identify novel circulating miRNAs as biomarkers for the early detection of CRC. Materials and Methods: Total 205 participants were enrolled in this study. First, two dysregulated candidate miRNAs were selected after integrated analysis of four GEO datasets. Then, the expression of these two miRNAs in plasma samples were tested through qRT-PCR. Training phase and validation phase were designed to verify the diagnostic value of these two miRNAs using receiver operating characteristic curve (ROC) analysis. Results: After integrated analysis of GEO datasets, we discovered miR-1290 and miR-320d were dysregulated in colorectal adenoma and adenocarcinoma tissues, and circulating miR-1290 and miR-320d in CRC patients were tumor-derived. Thereafter, circulating miR-1290 and miR-320d were selected to further investigate their potential for early diagnosis of CRC. Plasma miR-1290 expression could differentiate adenoma and CRC patients from healthy controls with area under the curve (AUC) of 0.78 and 0.88. Similarly, plasma miR-320d expression could discriminate adenoma and CRC patients from healthy controls with AUC of 0.74 and 0.81. Conclusions: Circulating miR-1290 and miR-320d are novel promising biomarkers for early diagnosis of CRC.

SUBMITTER: Liu X

PROVIDER: S-EPMC6329864 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Circulating miR-1290 and miR-320d as Novel Diagnostic Biomarkers of Human Colorectal Cancer.

Liu Xiangxiang X Xu Xueni X Pan Bei B He Bangshun B Chen Xiaoxiang X Zeng Kaixuan K Xu Mu M Pan Yuqin Y Sun Huiling H Xu Tao T Hu Xiuxiu X Wang Shukui S

Journal of Cancer 20190101 1

<b>Background</b>: The lack of screening methods with high diagnostic utility leads to colorectal cancer (CRC) patients usually diagnosed in advanced stages which results in high mortality. This study aimed to identify novel circulating miRNAs as biomarkers for the early detection of CRC. <b>Materials and Methods</b>: Total 205 participants were enrolled in this study. First, two dysregulated candidate miRNAs were selected after integrated analysis of four GEO datasets. Then, the expression of t ...[more]

PMID: 30662524

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Project description:BackgroundEarly detection of colorectal neoplasms, including colorectal cancers (CRCs) and advanced colorectal adenomas (AAs), is crucial to improve patient survival. Circulating microRNAs (miRNAs) in peripheral blood are emerging as noninvasive diagnostic markers for multiple cancers, but their potential for screening colorectal neoplasms remains ambiguous.AimTo identify candidate circulating cell-free miRNAs as diagnostic biomarkers in patients with colorectal neoplasms.MethodsThe study was divided into three phases: (1) Candidate miRNAs were selected from three public miRNA datasets using differential gene expression analysis methods; (2) an independent set of serum samples from 60 CRC patients, 60 AA patients and 30 healthy controls (HCs) was included and analyzed by quantitative real-time polymerase chain reaction for miRNAs, and their diagnostic power was detected by receiver operating characteristic (ROC) analysis; and (3) the origin and function of miRNAs in cancer patients were investigated in cancer cell lines and tumor tissues.ResultsBased on bioinformatics analysis, miR-627-5p and miR-199a-5p were differentially expressed in both the serum and tissues of patients with colorectal neoplasms and HCs and were selected for further study. Further validation in an independent cohort revealed that both circulating miR-627-5p and miR-199a-5p were sequentially increased from HCs and AAs to CRCs. The diagnostic power of miR-672-5p yielded an area under the curve (AUC) value of 0.90, and miR-199a-5p had an AUC of 0.83 in discriminating colorectal neoplasms from HCs. A logistic integrated model combining miR-199a-5p and miR-627-5p exhibited a higher diagnostic performance than either miRNA. Additionally, the levels of serum miR-627-5p and miR-199a-5p in CRC patients were significantly lower after surgery than before surgery and the expression of both miRNAs was increased with culture time in the culture media of several CRC cell lines, suggesting that the upregulated serum expression of both miRNAs in CRC might be tumor derived. Furthermore, in vitro experiments revealed that miR-627-5p and miR-199a-5p acted as tumor suppressors in CRC cells.ConclusionSerum levels of miR-199a-5p and miR-627-5p were markedly increased in patients with colorectal neoplasms and showed strong potential as minimally invasive biomarkers for the early screening of colorectal neoplasms.

Dataset Information

Circulating miR-1290 and miR-320d as Novel Diagnostic Biomarkers of Human Colorectal Cancer.

Publications

Circulating miR-1290 and miR-320d as Novel Diagnostic Biomarkers of Human Colorectal Cancer.

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