The Lec5 glycosylation mutant links homeobox genes with cholesterol and lipid-linked oligosaccharides.
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ABSTRACT:
SUBMITTER: Lu H
PROVIDER: S-EPMC6330018 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Publications
The Lec5 glycosylation mutant links homeobox genes with cholesterol and lipid-linked oligosaccharides.
Lu Hua H Sathe Adwait Amod AA Xing Chao C Lehrman Mark A MA
Glycobiology 20190201 2
Discovered 40 years ago, the Lec5 glycosylation mutant cell line has a complex recessive genotype and is characterized by accumulation of lipid-linked oligosaccharide assembly intermediates, reduced conversion of polyprenols to dolichols, and an unusual phenotypic dependence upon cell culture conditions such as temperature, plating density and medium quality. The heritable defect in Lec5 is unknown. Here we demonstrate an unexpected epigenetic basis for Lec5, with a surprising linkage to increas ...[more]
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