Ontology highlight
ABSTRACT:
SUBMITTER: Pedersen M
PROVIDER: S-EPMC6330563 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Pedersen Meike M Jamali Shirin S Saha Ipsita I Daum Rainer R Bendjennat Mourad M Saffarian Saveez S
European biophysics journal : EBJ 20180724 1
Interferometric Photo-Activation-Localization-Microscopy (iPALM) localizes single fluorescent molecules with 20 nm lateral and 10 nm axial resolution. We present a method utilizing glass coverslip lithography for correlative imaging between iPALM and scanning electron microscopy (SEM). Using iPALM on HIV Gag-Dendra virus-like particles (VLPs) we localized the position of HIV Gag proteins. Based on these localizations we reconstructed the central cavity of the VLPs along with imperfections within ...[more]