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Correlative iPALM and SEM resolves virus cavity and Gag lattice defects in HIV virions.

ABSTRACT: Interferometric Photo-Activation-Localization-Microscopy (iPALM) localizes single fluorescent molecules with 20 nm lateral and 10 nm axial resolution. We present a method utilizing glass coverslip lithography for correlative imaging between iPALM and scanning electron microscopy (SEM). Using iPALM on HIV Gag-Dendra virus-like particles (VLPs) we localized the position of HIV Gag proteins. Based on these localizations we reconstructed the central cavity of the VLPs along with imperfections within the HIV Gag lattice. The SEM images and iPALM images overlap and show imaging from single VLPs immobilized on glass coverslips. The localization of many HIV proteins including accessory proteins and Gag-Pol remains unknown, we discuss how the specificity of iPALM coupled with SEM has the potential for resolving more of HIV proteins.

SUBMITTER: Pedersen M

PROVIDER: S-EPMC6330563 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Correlative iPALM and SEM resolves virus cavity and Gag lattice defects in HIV virions.

Pedersen Meike M Jamali Shirin S Saha Ipsita I Daum Rainer R Bendjennat Mourad M Saffarian Saveez S

European biophysics journal : EBJ 20180724 1

Interferometric Photo-Activation-Localization-Microscopy (iPALM) localizes single fluorescent molecules with 20 nm lateral and 10 nm axial resolution. We present a method utilizing glass coverslip lithography for correlative imaging between iPALM and scanning electron microscopy (SEM). Using iPALM on HIV Gag-Dendra virus-like particles (VLPs) we localized the position of HIV Gag proteins. Based on these localizations we reconstructed the central cavity of the VLPs along with imperfections within ...[more]

PMID: 30043246

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