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Viral species richness and composition in young children with loose or watery stool in Ethiopia.

ABSTRACT: BACKGROUND:Stool consistency is an important diagnostic criterion in both research and clinical medicine and is often used to define diarrheal disease. METHODS:We examine the pediatric enteric virome across stool consistencies to evaluate differences in richness and community composition using fecal samples collected from children aged 0 to 5?years participating in a clinical trial in the Amhara region of Ethiopia. The consistency of each sample was graded according to the modified Bristol Stool Form Scale for children (mBSFS-C) before a portion of stool was preserved for viral metagenomic analysis. Stool samples were grouped into 29 pools according to stool consistency type. Differential abundance was determined using negative-binomial modeling. RESULTS:Of 446 censused children who were eligible to participate, 317 presented for the study visit examination and 269 provided stool samples. The median age of children with stool samples was 36?months. Species richness was highest in watery-consistency stool and decreased as stool consistency became firmer (Spearman's r?=?-?0.45, p?=?0.013). The greatest differential abundance comparing loose or watery to formed stool was for norovirus GII (7.64, 95% CI 5.8, 9.5) followed by aichivirus A (5.93, 95% CI 4.0, 7.89) and adeno-associated virus 2 (5.81, 95%CI 3.9, 7.7). CONCLUSIONS:In conclusion, we documented a difference in pediatric enteric viromes according to mBSFS-C stool consistency category, both in species richness and composition.

SUBMITTER: Aiemjoy K

PROVIDER: S-EPMC6332554 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Viral species richness and composition in young children with loose or watery stool in Ethiopia.

Aiemjoy Kristen K Altan Eda E Aragie Solomon S Fry Dionna M DM Phan Tung G TG Deng Xutao X Chanyalew Melsew M Tadesse Zerihun Z Callahan E Kelly EK Delwart Eric E Keenan Jeremy D JD

BMC infectious diseases 20190114 1

<h4>Background</h4>Stool consistency is an important diagnostic criterion in both research and clinical medicine and is often used to define diarrheal disease.<h4>Methods</h4>We examine the pediatric enteric virome across stool consistencies to evaluate differences in richness and community composition using fecal samples collected from children aged 0 to 5 years participating in a clinical trial in the Amhara region of Ethiopia. The consistency of each sample was graded according to the modifie ...[more]

PMID: 30642268

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Dataset Information

Viral species richness and composition in young children with loose or watery stool in Ethiopia.

Publications

Viral species richness and composition in young children with loose or watery stool in Ethiopia.

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