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Highly efficient gene release in spatiotemporal precision approached by light and pH dual responsive copolymers.


ABSTRACT: Triblock copolymer of poly(ethylene glycol)-b-poly(2-dimethylaminoethyl methacrylate)-b-poly(pyrenylmethyl methacrylate) (PEG-b-PDMAEMA-b-PPy) has been developed for use as an ideal gene delivery system, which showed both high stability under physiological conditions and efficient gene release in a mimetic cancer environment. The siRNA release from this system without external stimulation was 16% in 1 h and then remained steady. However, the photo-triggered siRNA release was 78% within 1 h and was higher than 91% after 24 h. The remarkable contrast between the stability and release efficiency of these siRNA-condensed micelleplexes before and after photo-irradiation has been rationalized by the light- and pH-induced structural transitions of the triblock copolymer micelles. The negligible cytotoxicity, high cellular uptake efficiency, and remarkable knockdown efficiency shown in in vitro tests further revealed the promising potential of these triblock copolymer micelleplexes for use in stimuli-responsive gene therapy.

SUBMITTER: Lu HH 

PROVIDER: S-EPMC6333234 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Highly efficient gene release in spatiotemporal precision approached by light and pH dual responsive copolymers.

Lu Hung-Hsun HH   Huang Cheng-Hung CH   Shiue Ting-Yun TY   Wang Fu-Sheng FS   Chang Ko-Kai KK   Chen Yunching Y   Peng Chi-How CH  

Chemical science 20181004 1


Triblock copolymer of poly(ethylene glycol)-<i>b</i>-poly(2-dimethylaminoethyl methacrylate)-<i>b</i>-poly(pyrenylmethyl methacrylate) (PEG-<i>b</i>-PDMAEMA-<i>b</i>-PPy) has been developed for use as an ideal gene delivery system, which showed both high stability under physiological conditions and efficient gene release in a mimetic cancer environment. The siRNA release from this system without external stimulation was 16% in 1 h and then remained steady. However, the photo-triggered siRNA rele  ...[more]

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