Dataset Information

Behavioral Impact of Long-Term Chronic Implantation of Neural Recording Devices in the Rhesus Macaque.

ABSTRACT: BACKGROUND:Ensemble recording methods are pervasive in basic and clinical neuroscience research. Invasive neural implants are used in patients with drug resistant epilepsy to localize seizure origin, in neuropsychiatric or Parkinson's patients to alleviate symptoms via deep brain stimulation, and with animal models to conduct basic research. Studies addressing the brain's physiological response to chronic electrode implants demonstrate that the mechanical trauma of insertion is followed by an acute inflammatory response as well as a chronic foreign body response. Despite use of invasive recording methods with animal models and humans, little is known of their effect on behavior in healthy populations. OBJECTIVE:To quantify the effect of chronic electrode implantation targeting the hippocampus on recognition memory performance. METHODS:Four healthy female rhesus macaques were tested in a delayed nonmatching-to-sample (DNMS) recognition memory task before and after hippocampal implantation with a tetrode array device. RESULTS:Trials to criterion and recognition memory performance were not significantly different before vs. after chronic electrode implantation. CONCLUSION:Our results suggest that chronic implants did not produce significant impairments on DNMS performance.

SUBMITTER: Kyle CT

PROVIDER: S-EPMC6336531 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Behavioral Impact of Long-Term Chronic Implantation of Neural Recording Devices in the Rhesus Macaque.

Kyle Colin T CT Permenter Michele R MR Vogt Julie A JA Rapp Peter R PR Barnes Carol A CA

Neuromodulation : journal of the International Neuromodulation Society 20180717 4

<h4>Background</h4>Ensemble recording methods are pervasive in basic and clinical neuroscience research. Invasive neural implants are used in patients with drug resistant epilepsy to localize seizure origin, in neuropsychiatric or Parkinson's patients to alleviate symptoms via deep brain stimulation, and with animal models to conduct basic research. Studies addressing the brain's physiological response to chronic electrode implants demonstrate that the mechanical trauma of insertion is followed ...[more]

PMID: 30016006

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Project description:The occurrence of clonal perturbations and leukemia in patients transplanted with retrovirally-transduced autologous hematopoietic stem and progenitor cells (HSPCs) has stimulated extensive investigation, demonstrating that proviral insertions perturb adjacent proto-oncogene expression. Although enhancer-deleted lentiviruses are less likely to result in insertional oncogenesis, there is evidence that they may perturb transcript splicing, and one patient with a benign clonal expansion of lentivirally-transduced HPSC has been reported. The rhesus macaque model provides an opportunity for informative long-term analysis to ask whether transduction impacts on long-term HSPC properties. We utilized two techniques to examine whether lentivirally-transduced HSPCs from eight rhesus macaques transplanted 1-13.5 years previously are perturbed at a population level, comparing telomere length as a measure of replicative history and gene expression profile of vector positive versus vector negative cells. There were no differences in telomere lengths between sorted GFP+ and GFP- blood cells, suggesting that lentiviral transduction did not globally disrupt replicative patterns. Bone marrow GFP+ and GFP- CD34+ cells showed no differences in gene expression using unsupervised and principal component analysis. These studies did not uncover any global long-term perturbation of proliferation, differentiation, or other important functional parameters of transduced HSPCs in the rhesus macaque model. CD34+ hematopoietic stem and progenitor cells were purified from bone marrow aspirates obtained from 4 rhesus macaques 3-9 years following transplantation with lentivirally-transduced autologous hematopoietic stem and progenitor cells. All lentiviral vectors contained the GFP marker gene. The CD34+ cells from each animal were sorted via flow cytometry into GFP+ and GFP- fractions, and RNA from these cells was used for Affymetrix gene expression analysis as detailed below. There were two samples, GFP+ and GFP-, from each of 4 animals.

Dataset Information

Behavioral Impact of Long-Term Chronic Implantation of Neural Recording Devices in the Rhesus Macaque.

Publications

Behavioral Impact of Long-Term Chronic Implantation of Neural Recording Devices in the Rhesus Macaque.

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