Ontology highlight
ABSTRACT:
SUBMITTER: Jia H
PROVIDER: S-EPMC6337133 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Jia Hongmin H Zhou Tianjiao T Zhu Hong H Shen Li L He Pingli P
Molecules (Basel, Switzerland) 20181225 1
Gly m 5.0101, the alpha subunit of β-conglycinin, is one of the major allergens found in soybeans that has been identified as causing an allergic reaction. Here, we developed a quantification method of Gly m 5.0101 with multiple reaction monitoring using the synthetic peptide <sup>194</sup>NPFLFGSNR<sup>202</sup> as the external standard. Firstly, the ground soybean was defatted and extracted with a protein extraction buffer. Then the crude extract was on-filter digested by trypsin and analyzed ...[more]