Ontology highlight
ABSTRACT:
SUBMITTER: Paidikondala M
PROVIDER: S-EPMC6337215 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Paidikondala Maruthibabu M Kadekar Sandeep S Varghese Oommen P OP
International journal of molecular sciences 20181223 1
Ex vivo gene therapy offers enormous potential for cell-based therapies, however, cumbersome in vitro cell culture conditions have limited its use in clinical practice. We have optimized an innovative strategy for the transient transfection of bone morphogenetic protein-2 (BMP-2) expressing plasmids in suspended human stem cells within 5-min that enables efficient loading of the transfected cells into a 3D hydrogel system. Such a short incubation time for lipid-based DNA nanoparticles (lipoplexe ...[more]